sksbio

The Validation of the Wheat Gluten ELISA Kit.

Background: It is very important analyze the presence of wheat/gluten in meals to keep away from wheat allergy or celiac illness.

Goal: The Wheat/Gluten ELISA equipment was developed to measure complete wheat protein or gluten content material in wheat, barley, and rye cereals as uncooked supplies, and processed meals. Validation as as to whether this equipment is appropriate for quantifying complete wheat protein/gluten was carried out.

Strategies: The Wheat/Gluten ELISA equipment was designed as a sandwich ELISA based mostly on antigliadin polyclonal antibody. Selectivity, interference examine, matrix examine together with incurred meals, robustness, stability, and lot-to-lot consistency research have been carried out for the Wheat/Gluten ELISA equipment. Incurred matrix research have been additionally carried out in an impartial laboratory.

Outcomes: The evaluation of 38 completely different substances revealed no cross-reactivity above the LOQ apart from oats. Recoveries of the spiked samples have been principally within the vary of 75-140%, together with an impartial laboratory end result. The LOD of the ELISA was discovered to be 0.02-0.16 mg/kg. Robustness testing proved that extraction time and incubation time of first response and enzyme response had no important affect on quantified worth. The steadiness at 2-8°C was discovered to exceed 12 months. Good lot-to-lot consistency was noticed.

Conclusions: The Wheat/Gluten ELISA equipment confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten within the recognized meals merchandise utilizing the AOAC Efficiency Examined Technique(s)SM program.

Highlights: The Wheat/Gluten ELISA equipment was validated and confirmed good analytical efficiency within the quantitative evaluation of complete wheat protein/gluten in meals.

Inter-laboratory comparability of two ELISA kits used for foot-and-mouth illness virus nonstructural protein serology

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth illness virus (FMDV) are used for illness surveillance in endemic international locations, and are important to offering proof for freedom of the illness with or with out vaccination and to recuperate the free standing of a rustic after an outbreak. In a 5-site inter-laboratory examine, we in contrast the efficiency of two business NSP ELISA kits (ID Display screen FMD NSP ELISA single day [short] and in a single day protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The general concordance between the PrioCHECK and ID Display screen check was 93.8% (95% CI: 92.0-95.2%) and 94.8% (95% CI: 93.1-96.1%) for the in a single day and brief ID Display screen incubation protocols, respectively. Our outcomes point out that the assays (together with the two completely different codecs of the ID Display screen check) can be utilized interchangeably in post-outbreak serosurveillance.

 

Protecting impact of overexpression of PrxII on H2O2-induced cardiomyocyte damage

 

Goal: Oxidative stress is without doubt one of the important components resulting in myocardial cell harm, and the redox imbalance promotes apoptosis. Due to this fact, the aim of this examine was to discover the protecting impact of PrxII on H2O2-induced H9c2 cell damage.

 

Supplies and strategies: The overexpressed PrxII cell mannequin was constructed by virus. The H9c2 cells have been handled with H2O2, and the supernatant and cells have been collected. Then, the chymotrypsin-like exercise, caspase-like exercise, and trypsin-like exercise have been detected by the equipment, and the expressions of P21, P27, and P53 have been detected by the ELISA technique. Lastly, the expressions of antioxidant components, apoptosis-related components, and AMPK/Sirt1 signaling pathway have been detected by Western blot and Actual-time polymerase chain response (PCR).

 

Outcomes: Overexpression of PrxII inhibited the lower of enzyme exercise induced by H2O2, promoted the expressions of anti-oxidation components GPX1, GPX2, and GSX, and inhibited the expressions of apoptosis-related components P21, P27, and P53, and activated AMPK/Sirt1 pathway.

sksbio
sksbio

 

Analysis of toxoplasmosis in pregnant girls utilizing dot-immunogold-silver staining with recombinant Toxoplasma gondii peroxiredoxin protein

 

Background: Toxoplasma gondii an infection endangers human well being and impacts animal husbandry. Serological detection is the principle technique used for epidemiological investigations and analysis of toxoplasmosis. The important thing to efficient analysis of toxoplasmosis is the usage of a standardized antigen and a particular and delicate detection technique. Peroxiredoxin is an antigenic protein and vaccine candidate antigen of T. gondii that has not but been exploited for diagnostic utility.

 

Strategies: On this examine, recombinant T. gondii peroxiredoxin protein (rTgPrx) was ready and utilized in dot-immunogold-silver staining (Dot-IGSS) to detect IgG antibodies in serum from mice and pregnant girls. The rTgPrx-Dot-IGSS technique was established and optimized utilizing mouse serum. Moreover, serum samples from pregnant girls have been analyzed by rTgPrx-Dot-IGSS.

 

Outcomes: Forty serum samples from mice contaminated with T. gondii and twenty unfavourable serum samples have been analyzed. The sensitivity and specificity of rTgPrx-Dot-IGSS have been 97.5 and 100%, respectively, equal to these of a business ELISA equipment for anti-Toxoplasma IgG antibody. Moreover, 540 serum samples from pregnant girls have been screened with a business ELISA equipment. Eighty-three constructive and 60 unfavourable serum samples have been analyzed by rTgPrx-Dot-IGSS. The constructive charge was 95.18%, corresponding to that obtained with the business ELISA equipment.

 

Conclusions: The Dot-IGSS technique with rTgPrx as an antigen may be helpful for diagnosing T. gondii an infection in people.

 

Dedication of antibody induction by extremely pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) vaccine: A comparability of two ELISA kits.

 

Two business porcine reproductive and respiratory syndrome virus (PRRSV) antibody ELISA kits (IDEXX and LSI) are presently in intensive use. To find out which equipment is extra appropriate for the analysis of HP-PRRSV vaccine efficacy, the 2 kits have been used to check 546 serum samples. The settlement between the outcomes was unsatisfactory, with a kappa statistic of 0.681 and a linear correlation coefficient of 0.665. In assessments of samples from experimentally vaccinated and PRRSV-negative herds, IDEXX-ELISA recognized antibody-positive conversion earlier and confirmed the next specificity in comparison with LSI-ELISA. The serological profile obtained by neutralization testing was nearer to that obtained by IDEXX-ELISA than by LSI-ELISA within the late immunization interval. The findings reveal that IDEXX-ELISA is the extra appropriate for the analysis of antibody response to HP-PRRSV vaccine and for guiding vaccination methods.
Background Therapeutic drug monitoring (TDM) of adalimumab (ADA) in inflammatory bowel illnesses (IBDs) has gained elevated consideration since a number of research confirmed a correlation between drug ranges and mucosal therapeutic. The constraints of routine utilization of enzyme-linked immunoabsorbent assay (ELISA) kits for measuring serum ADA concentrations have prompted the event of fast strategies, comparable to Quantum Blue (QB). We evaluated the interchangeability and settlement between the QB technique and two established ELISA kits, Promonitor (PM) and Lisa-Tracker (LT). Strategies Fifty samples from sufferers with IBD have been included.
Quantitative evaluation was carried out utilizing the ANOVA check for repeated measures, Deming regression and the Bland-Altman plot. Medical implications have been evaluated by concordance in classifying sufferers into therapeutic home windows in line with the proposed cut-off ranges for subtherapeutic (both <5 or <7.5 μg/mL) and supratherapeutic (>12 μg/mL) ranges. Outcomes Statistical variations have been detected between the QB technique and the 2 ELISA kits, with QB overestimating ADA serum values in comparison with them.
An absence of interchangeability was noticed between strategies, with larger variations as ADA ranges elevated. An evaluation of a sub-set of samples with ADA values beneath 9 μg/mL (n = 25) confirmed that QB fulfilled the factors to be interchangeable with the LT assay. Concordance for affected person classification into ADA therapeutic home windows was higher for QB vs. LT than for QB vs. PM, with excessive settlement (>75%) for subtherapeutic ranges among the many three strategies. Conclusions Though quantitative variations existed between the fast technique and ELISA kits that hampered their interchangeability, the settlement for figuring out sufferers with subtherapeutic values of ADA was excessive.

Human Myoglobin ELISA Kit

MBS006477-96StripWells 96-Strip-Wells
EUR 765

Human Myoglobin ELISA Kit

IHUMYOKT each
EUR 431
Description: Human Myoglobin ELISA Kit

Human Myoglobin ELISA Kit

IHUMBKT each
EUR 538
Description: Human Myoglobin ELISA Kit

Human Myoglobin ELISA Kit

GWB-AF81CB 96 well Ask for price

Human Myoglobin ELISA Kit

MBS9425687-5x96Tests 5x96Tests
EUR 2070

Human Myoglobin ELISA Kit

MBS9425687-96Tests 96Tests
EUR 455

Human Myoglobin ELISA Kit

MBS763854-10x96StripWells 10x96-Strip-Wells
EUR 3900

Human Myoglobin ELISA Kit

MBS763854-48StripWells 48-Strip-Wells
EUR 340

Human Myoglobin ELISA Kit

MBS763854-5x96StripWells 5x96-Strip-Wells
EUR 2045

Human Myoglobin ELISA Kit

MBS763854-96StripWells 96-Strip-Wells
EUR 455

Human Myoglobin ELISA Kit

MBS732828-48StripWellsCompetitive 48-Strip-Wells-(Competitive)
EUR 485

Human Myoglobin ELISA Kit

MBS732828-48StripWellsSandwich 48-Strip-Wells-(Sandwich)
EUR 485

Human Myoglobin ELISA Kit

MBS732828-5x96StripWellsCompetitive 5x96-Strip-Wells-(Competitive)
EUR 3020

Human Myoglobin ELISA Kit

MBS732828-96StripWellsCompetitive 96-Strip-Wells-(Competitive)
EUR 690

Human Myoglobin ELISA Kit

MBS732828-96StripWellsSandwich 96-Strip-Wells-(Sandwich)
EUR 690

Human Myoglobin ELISA Kit

MBS8579474-5x96Tests 5x96Tests
EUR 3190

Human Myoglobin ELISA Kit

MBS8579474-96Tests 96Tests
EUR 695

Human Myoglobin ELISA Kit

MBS8415018-1Kit 1Kit
EUR 760

Human Myoglobin ELISA Kit

MBS8415018-5x1Kit 5x1Kit
EUR 3480

Human Myoglobin ELISA Kit

MBS289896-10x96StripWells 10x96-Strip-Wells
EUR 3290

Human Myoglobin ELISA Kit

MBS289896-48StripWells 48-Strip-Wells
EUR 340

Human Myoglobin ELISA Kit

MBS289896-5x96StripWells 5x96-Strip-Wells
EUR 1800

Human Myoglobin ELISA Kit

MBS289896-96StripWells 96-Strip-Wells
EUR 435

Human Myoglobin (MB) ELISA Kit

20-abx152437
  • Ask for price
  • Ask for price
  • Ask for price
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Myoglobin (MB) ELISA Kit

abx252858-96tests 96 tests
EUR 764.4

Human Myoglobin (MB) ELISA Kit

abx573468-96tests 96 tests
EUR 764.4

Human MB/ Myoglobin ELISA Kit

E2800Hu 1 Kit
EUR 515

Human Myoglobin (MB) ELISA Kit

AE34161HU-48Tests 48 Tests
EUR 360
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

AE34161HU-96Tests 96 Tests
EUR 680
Description: Human (Homo sapiens)

Human MB / Myoglobin ELISA Kit

E0480h 96T
EUR 536

Human Myoglobin,MB ELISA KIT

E1241Hu-1096T 10*96T
EUR 4122

Human Myoglobin,MB ELISA KIT

E1241Hu-48wells 48 wells
EUR 300

Human Myoglobin,MB ELISA KIT

E1241Hu-596T 5*96T
EUR 2061

Human Myoglobin,MB ELISA KIT

E1241Hu-96wells 96 wells
EUR 458

Human Myoglobin (MB) ELISA Kit

KTE61705-48T 48T
EUR 359
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T 96T
EUR 619
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T5 96 T×5
EUR 2809
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

KTE61705-96T50 96 T×50
EUR 26209
Description: This Human Myoglobin (MB) ELISA Kit employs a two-site sandwich ELISA to quantitate MB.

Human Myoglobin (MB) ELISA Kit

RK09255 96T
EUR 280

Human Myoglobin (MB) ELISA Kit

AE34161HU-48T 48T
EUR 360
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

AE34161HU-96T 96T
EUR 680
Description: Human (Homo sapiens)

Human Myoglobin (MB) ELISA Kit

MBS9715369-48Tests 48Tests
EUR 350

Human Myoglobin (MB) ELISA Kit

MBS9715369-5x96Tests 5x96Tests
EUR 1940

Human Myoglobin (MB) ELISA Kit

MBS9715369-96Tests 96Tests
EUR 520

Human Myoglobin ELISA Kit (MYO)

MBS9137438-5x96Tests 5x96Tests
EUR 2615

Human Myoglobin ELISA Kit (MYO)

MBS9137438-96Tests 96Tests
EUR 570

Human Myo(Myoglobin) ELISA Kit

EH0347 96T
EUR 571.5
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.938 ng/ml

Human Myo(Myoglobin) ELISA Kit

EKF57132-48T 48T
EUR 396.9

Human Myo(Myoglobin) ELISA Kit

EKF57132-5x96T 5x96T
EUR 2693.25

Human Myo(Myoglobin) ELISA Kit

EKF57132-96T 96T
EUR 567

Human MYO (Myoglobin) ELISA Kit

EKE60124-5x96T 5x96T
EUR 2667.6

Human MYO (Myoglobin) ELISA Kit

EKE60124-96T 96T
EUR 561.6

Human Myo(Myoglobin) ELISA Kit

E39EH0347 96T
EUR 595

Human MYO(Myoglobin) ELISA Kit

ELK1211-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

Human MYO(Myoglobin) ELISA Kit

ELK1211-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYO in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Myo(Myoglobin) ELISA Kit

ELK1357-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Myo(Myoglobin) ELISA Kit

ELK1357-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Myo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Myo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Myo, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Myo in the samples is then determined by comparing the OD of the samples to the standard curve.

Human MYO(Myoglobin) ELISA Kit

RE2746H-48wells 48 wells
EUR 116.55

Human MYO(Myoglobin) ELISA Kit

RE2746H-96wells 96 wells
EUR 161.55

Human MYO (Myoglobin) ELISA Kit

MBS8800213-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human MYO (Myoglobin) ELISA Kit

MBS8800213-48StripWells 48-Strip-Wells
EUR 315

Human MYO (Myoglobin) ELISA Kit

MBS8800213-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human MYO (Myoglobin) ELISA Kit

MBS8800213-96StripWells 96-Strip-Wells
EUR 385

Human Myo (Myoglobin) ELISA Kit

MBS8800359-10x96StripWells 10x96-Strip-Wells
EUR 3130

Human Myo (Myoglobin) ELISA Kit

MBS8800359-48StripWells 48-Strip-Wells
EUR 315

Human Myo (Myoglobin) ELISA Kit

MBS8800359-5x96StripWells 5x96-Strip-Wells
EUR 1710

Human Myo (Myoglobin) ELISA Kit

MBS8800359-96StripWells 96-Strip-Wells
EUR 385

Human MYO (Myoglobin) ELISA Kit

MBS2567291-10x96Tests 10x96Tests
EUR 3265

Human MYO (Myoglobin) ELISA Kit

MBS2567291-24Tests 24Tests
EUR 225

Human MYO (Myoglobin) ELISA Kit

MBS2567291-48Test 48Test
EUR 350

Human MYO (Myoglobin) ELISA Kit

MBS2567291-5x96Test 5x96Test
EUR 1655

Human MYO (Myoglobin) ELISA Kit

MBS2567291-96Tests 96Tests
EUR 395

Human MYO (Myoglobin) ELISA Kit

MBS2515596-10x96Tests 10x96Tests
EUR 3265

Human MYO (Myoglobin) ELISA Kit

MBS2515596-24Tests 24Tests
EUR 225

Human MYO (Myoglobin) ELISA Kit

MBS2515596-48Test 48Test
EUR 350

Human MYO (Myoglobin) ELISA Kit

MBS2515596-5x96Test 5x96Test
EUR 1655

Human MYO (Myoglobin) ELISA Kit

MBS2515596-96Tests 96Tests
EUR 395

Myoglobin (Human) ELISA Kit

E4326-100 each
EUR 874.8

Nori® Human Myoglobin ELISA Kit

GR111252 96-well
EUR 461

Myoglobin ELISA Kit (Human) (OKIA00078)

OKIA00078 96 Wells
EUR 606
Description: Description of target: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. ;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.540 ng/ml

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu 96T
EUR 272
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu-48T 48T
EUR 414
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DLR-MYO-Hu-96T 96T
EUR 524.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Myoglobin (MYO) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

DL-MYO-Hu 96T
EUR 259
Description: serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Human Myoglobin (MYO) ELISA Kit

EKL59275-5x96T 5x96T
EUR 2229.18

Human Myoglobin (MYO) ELISA Kit

EKL59275-96T 96T
EUR 469.3

Human Myoglobin(MYO) Elisa Kit

EK711221 96 Wells
EUR 0.15

Human Myoglobin (MYO) ELISA Kit

EKN47189-48T 48T
EUR 205.38

Human Myoglobin (MYO) ELISA Kit

EKN47189-5x96T 5x96T
EUR 1393.65

Human Myoglobin (MYO) ELISA Kit

EKN47189-96T 96T
EUR 293.4

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