This SARS-CoV-2 IgG Seroconversion ELISA Kit from Innovative Research is designed for the qualitative determination of SARS-CoV-2 specific IgG class antibodies in plasma or serum samples. Peel well format. Reagents for up to 96 tests. For research only.

This assay has been specifically developed using the SARS-CoV-2 receptor-binding domain (RBD) of the spike protein. This provides reliable and consistent results with a high degree of reproducibility. This assay can be performed in 70 minutes and has an overall sensitivity of 97-100% and a specificity of 100%.

Test principle:

SARS-CoV-2 specific antibodies will bind to the receptor-binding domain (RBD) derived from purified recombinant HEK cells from the SARS-CoV-2 peak protein coated on the microtiter plate. After the appropriate washing steps, horseradish peroxidase-labeled polyclonal anti-human IgG secondary antibody binds to the captured protein. The excess secondary antibody is washed away and the TMB substrate is used for colour development at 450 nm. Samples that exceed a certain cut-off value are termed positive in this test.

More details:

Detection Target: IgG isotype-specific antibodies against SARS-CoV-2

Sample dilution: A 1:51 dilution of serum samples is suggested for best results.

Expected Values: Significant seroconversion, demonstrated by antibodies specific for both the SARS-CoV-2 full-length peak protein and the RBD region, has been detected as early as two days after the onset of symptoms.

Sensitivity: 97% for samples obtained more than 14 days after the onset of symptoms (n = 37), 100% for samples obtained more than 21 days after the onset of symptoms (n = 32).

Specificity: 100% (n = 89)

Percentage Agreement: Positive 100%; Negative 91%

Storage: Store all kit components at 4 ° C upon arrival. Return the unused microplate strips to the desiccant plate bag. Reconstituted controls can be stored at -80 ° C for later use. Store all other unused kit components at 4 ° C. This kit should not be used beyond the expiration date.

Negative results do not exclude acute SARS-CoV-2 infection. If acute infection is suspected, direct testing for SARS-CoV-2 is necessary. Antibody test results should not be used to diagnose or exclude an acute SARS-CoV-2 infection. Positive results may be due to past or present infection with coronavirus strains other than SARS-CoV-2, such as KHU1, NL63, OC43, or 229E. This serological assay can be used for applications such as characterizing immune responses to viral infection by determining the presence of antibodies specific to viral antigen in sera from recovered and infected patients. At Innovative Research, we provide reliable and consistent products that deliver reliable and consistent results.