Absolutely built-in speedy microfluidic system translated from typical 96-well ELISA equipment
- To do this, a programmable and disposable on-chip pump and valve had been built-in on the system for exact management and actuation of microfluidic reagents, which regulated a response time and reagent quantity to assist the optimized protocols of ELISA.
- Because of the on-chip pump and valve, ELISA could possibly be executed with lowered consumption of reagents and shortening the assay time, that are essential for typical ELISA utilizing 96-well microplate.
- To reveal extremely delicate detection and easy-to-use operation, this unconventional system was efficiently utilized for the quantification of cardiac troponin I (cTnI) of 4.88 pg/mL utilizing a minimal pattern quantity of 30 µL with a shorter assay time of 15 min for every ELISA step.
- The restrict of detection (LOD) thus obtained was considerably improved than the standard 96-well platform.
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