Rosmarinic Acid, a Bioactive Phenolic Compound, Inhibits Glutamate Release from Rat Cerebrocortical Synaptosomes through GABA A Receptor Activation

Rosmarinic Acid, a Bioactive Phenolic Compound, Inhibits Glutamate Release from Rat Cerebrocortical Synaptosomes through GABA A Receptor Activation

Rosmarinic acid, a serious part of rosemary, is a polyphenolic compound with potential neuroprotective results. Asreducing the synaptic launch of glutamate is essential to reaching neuroprotectant’s pharmacotherapeutic results, the impact of rosmarinic acid on glutamate launch was investigated in rat cerebrocortical nerve terminals (synaptosomes). Rosmarinic acid depressed the 4-aminopyridine (4-AP)-induced glutamate launch in a concentration-dependent method. The removing of extracellular calcium and the blockade of vesicular transporters prevented the inhibition of glutamate launch by rosmarinic acid.

Rosmarinic acid decreased 4-AP-induced intrasynaptosomal Ca2+ elevation. The inhibition of N-, P/Q-type Ca2+ channels and the calcium/calmodulin-dependent kinase II (CaMKII) prevented rosmarinic acid from having results on glutamate launch. Rosmarinic acid additionally decreased the 4-AP-induced activation of CaMKII and the next phosphorylation of synapsin I, the primary presynaptic goal of CaMKII. As well as, immunocytochemistry confirmed the presence of GABAA receptors. GABAA receptor agonist and antagonist blocked the inhibitory impact of rosmarinic acid on 4-AP-evoked glutamate launch.

Docking knowledge additionally revealed that rosmarinic acid shaped a hydrogen bond with the amino acid residues of GABAA receptor. These outcomes instructed that rosmarinic acid prompts GABAA receptors in cerebrocortical synaptosomes to lower Ca2+ inflow and CaMKII/synapsin I pathway to inhibit the evoked glutamate launch.

Encapsulation of bioactive compounds from byproducts of two species of passionflowers: analysis of the physicochemical properties and managed launch in a gastrointestinal mannequin

This examine aimed to guage the discharge of energetic parts with antioxidant and antihypertensive capability from encapsulated extracts of the peel and seeds of Gulupa (Passiflora edulis f. edulis) and Cholupa (Passiflora maliformis) in an in vitro gastrointestinal digestion mannequin. Microencapsulated extracts have been ready with enzymatically modified rice starch because the encapsulating materials and ethanol extracts of seeds and peel of P. edulis f. edulis and P. maliformis as encapsulated materials. Microcapsule characterization was carried out by scanning electron microscopy with values of 4.54-5.13 μm and ξ potential values of -6.34 mV and -6.66 mV.

Dynamic gentle scattering (DLS) evaluation was performed with polydispersion values from 1.33 to 1.51, and dispersion stability evaluation was additionally performed. The overall phenol content material and antioxidant actions (ABTS, DPPH, and FRAP) and ACE inhibitory exercise (in vitro antihypertensive exercise) have been evaluated after every stage of digestion, with values higher than 80% of exercise earlier than gastrointestinal transit and with values higher than 55% exercise after the top of gastrointestinal transit. Gastrointestinal analysis of the encapsulated extracts was carried out with an ex vivo mannequin utilizing pig intestines and simulating the circumstances of digestion in three phases: the gastric (pH 2.Zero with 1.

Zero M HCl +0.5 g/L pepsin), enteric (pH 8.Zero with Krebs resolution +1.Zero mL/L bile) and last enteric (pH 7.5 Krebs resolution solely) phases. The microencapsulation of passionflower extracts confirmed good conduct in opposition to adjustments in pH and enzymatic actions all through digestion, thus selling a managed launch and focused supply of bioactive compounds, present process a paracellular mechanism by means of the intestinal barrier to protect the antioxidant exercise and ACE inhibitory that was proven by the extracts earlier than encapsulation of the fabric.

Rosmarinic Acid, a Bioactive Phenolic Compound, Inhibits Glutamate Release from Rat Cerebrocortical Synaptosomes through GABA A Receptor Activation

The impact of UV radiation on O-7 Actinomycete in producing bioactive compounds in numerous development circumstances

Actinomycetes have been recognized as an origin of many secondary metabolites, antibiotics and energetic parts that influence microbial development. Mediated mutations utilizing UV in follow for the breeding of organisms. The target of this examine is to analyses the influence of UV radiation on the (O-7) Actinomycete isolate. This was a potential analytical examine of a a number of of actinomycetes. The isolates have been screened for antimicrobial efficacy in opposition to a number of Gram-positive, Gram-negative micro organism, yeast, and fungi. Varied elements resembling UV, temperature, pH, gentle, agitation, fermentation durations and aeration have additionally been boosted for optimum antimicrobial manufacturing.

The isolate (O-7) Actinomycete has been acknowledged as a extremely bioactive producing organism. The isolate was uncovered to numerous wavelengths, instances underneath quite a few development circumstances. It was discovered that 4% focus of glucose as a carbon supply is considerably optimum for the manufacturing of antibiotic for (O-7) UV uncovered pressure, nonetheless, focus of 1% of lactose is considerably optimum for the manufacturing of antibiotic for (O-7) UV uncovered pressure. Yeast extract at a focus of 1% was discovered to be the very best supply of nitrogen for (O-7) UV uncovered, whereas pH 7.

Zero was discovered to be probably the most appropriate for a similar isolate. From the temperature optimization examine, it was noticed that (O-7) uncovered pressure confirmed good development and most antibiotic manufacturing at 28 °C. The soil-isolated organic compounds (O-7) have been efficient in opposition to sure sorts of micro organism and fungi, and the analysis additionally demonstrated that publicity to UV radiation enhanced the manufacturing of those compounds.Elucidating the switch of bioactive compounds between crops is important for understanding plant-plant interactions, growing pure pesticides and understanding their modes of motion. This text is protected by copyright. All rights reserved.

Translocation of metabolites between completely different plant species gives necessary hints in understanding the destiny of bioactive root exudates. Within the current examine, focused and untargeted mass spectrometry-based metabolomics was utilized to elucidate the switch of bioactive compounds between rye and a number of other crops and weed species. Our outcomes demonstrated that benzoxazinoids (BXs) synthesized by rye have been taken up by roots of neighbouring plant species and translocated into their shoots.

GPAT2 Recombinant Protein (Mouse)

RP139238 100 ug Ask for price

Gpat2 ORF Vector (Mouse) (pORF)

ORF046414 1.0 ug DNA
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GPAT2 ORF Vector (Human) (pORF)

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Gpat2 ORF Vector (Rat) (pORF)

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Gpat2 sgRNA CRISPR Lentivector set (Rat)

K6080101 3 x 1.0 ug
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Gpat2 sgRNA CRISPR Lentivector set (Mouse)

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GPAT2 sgRNA CRISPR Lentivector set (Human)

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Gpat2 sgRNA CRISPR Lentivector (Rat) (Target 1)

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Gpat2 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6080103 1.0 ug DNA
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Gpat2 sgRNA CRISPR Lentivector (Rat) (Target 3)

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Gpat2 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3673902 1.0 ug DNA
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Gpat2 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3673903 1.0 ug DNA
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Gpat2 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3673904 1.0 ug DNA
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GPAT2 sgRNA CRISPR Lentivector (Human) (Target 1)

K2772902 1.0 ug DNA
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GPAT2 sgRNA CRISPR Lentivector (Human) (Target 2)

K2772903 1.0 ug DNA
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GPAT2 Protein Vector (Human) (pPB-C-His)

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GPAT2 Protein Vector (Human) (pPB-N-His)

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GPAT2 Protein Vector (Rat) (pPB-C-His)

PV270906 500 ng
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GPAT2 Protein Vector (Rat) (pPB-N-His)

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GPAT2 Protein Vector (Mouse) (pPB-C-His)

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EUR 1065

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GPAT2 Protein Vector (Mouse) (pPM-C-HA)

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GPAT2 Protein Vector (Mouse) (pPM-C-His)

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GPAT2 3'UTR GFP Stable Cell Line

TU059103 1.0 ml
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GPAT2 3'UTR Luciferase Stable Cell Line

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Gpat2 3'UTR GFP Stable Cell Line

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Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

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Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

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Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

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Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

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Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K3673906 1.0 ug DNA
EUR 167

Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K3673907 1.0 ug DNA
EUR 167

Gpat2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K3673908 1.0 ug DNA
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GPAT2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K2772906 1.0 ug DNA
EUR 167

GPAT2 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

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CD11b Antibody Antibody

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H2B Antibody Antibody

AF4659 200ul
EUR 376
Description: H2B Antibody Antibody detects endogenous levels of H2B.

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 100 ug
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Antibody

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Anti-Anti-SEPT2 Antibody antibody

STJ25475 100 µl
EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ25477 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-SEPT8 Antibody antibody

STJ25479 100 µl
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT2 Antibody antibody

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Anti-Anti-SEPT7 Antibody antibody

STJ28963 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT8 Antibody antibody

STJ117206 100 µl
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-SEPT12 Antibody antibody

STJ117759 100 µl
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Description: This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.

Anti-Anti-MARCH6 Antibody antibody

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Anti-Anti-MARCH6 Antibody antibody

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Anti-Anti-MARCH7 Antibody antibody

STJ118752 100 µl
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Anti-Anti-SEPT3 Antibody antibody

STJ118990 100 µl
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Anti-Anti-SEPT1 antibody antibody

STJ119580 100 µl
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis and the maintenance of cellular morphology. This gene encodes a protein that can form homo- and heterooligomeric filaments, and may contribute to the formation of neurofibrillary tangles in Alzheimer's disease. Alternatively spliced transcript variants have been found but the full-length nature of these variants has not been determined. [provided by RefSeq, Dec 2012]

Anti-Anti-SEPT7 Antibody antibody

STJ116214 100 µl
EUR 277
Description: This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19.

Anti-Anti-SEPT6 antibody antibody

STJ11100949 100 µl
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Description: This gene is a member of the septin family of GTPases. Members of this family are required for cytokinesis. One version of pediatric acute myeloid leukemia is the result of a reciprocal translocation between chromosomes 11 and X, with the breakpoint associated with the genes encoding the mixed-lineage leukemia and septin 2 proteins. This gene encodes four transcript variants encoding three distinct isoforms. An additional transcript variant has been identified, but its biological validity has not been determined.

Anti-Anti-SEPT9 Antibody antibody

STJ111369 100 µl
EUR 277
Description: This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.

Anti-Anti-SEPT11 Antibody antibody

STJ111530 100 µl
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Anti-Anti-SEPT4 Antibody antibody

STJ112276 100 µl
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Description: This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is highly expressed in brain and heart. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. One of the isoforms (known as ARTS) is distinct; it is localized to the mitochondria, and has a role in apoptosis and cancer.

Anti-Anti-MARCH9 Antibody antibody

STJ112609 100 µl
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Anti-Anti-SEPT11 Antibody antibody

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EUR 277

Anti-Anti-SEPT11 Antibody antibody

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EUR 277

Anti-Anti-SEPT5 Antibody antibody

STJ114819 100 µl
EUR 277
Description: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

Anti-Anti-MARCH8 Antibody antibody

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Ly1 Antibody Reactive (LYAR) Antibody

20-abx123734
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Anti-Glycoprotein Antibody (GP) Antibody

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  • 1 mg
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Anti-Glycoprotein Antibody (GP) Antibody

20-abx319901
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Anti-Glycoprotein Antibody (GP) Antibody

20-abx319905
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
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Anti-Glycoprotein Antibody (GP) Antibody

20-abx319913
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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  • 20 ug
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Ly1 Antibody Reactive (LYAR) Antibody

20-abx311665
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
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  • 100 ug
  • 1 mg
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  • 20 ug
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Anti-Glycolipid Antibody (AGA) Antibody

20-abx004855
  • EUR 411.00
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Anti-Glycolipid Antibody (AGA) Antibody

abx036399-100ug 100 ug
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Ly1 Antibody Reactive (LYAR) Antibody

20-abx008109
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
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Ly1 Antibody Reactive (LYAR) Antibody

20-abx014333
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
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Ly1 Antibody Reactive (LYAR) Antibody

abx033330-400ul 400 ul
EUR 523

Ly1 Antibody Reactive (LYAR) Antibody

abx033330-80l 80 µl
EUR 286

Ly1 Antibody Reactive (LYAR) Antibody

abx234901-100ug 100 ug
EUR 551

Anti-Glycolipid Antibody (AGA) Antibody

abx230204-100ug 100 ug
EUR 481

Ly1 Antibody Reactive (LYAR) Antibody

20-abx324434
  • EUR 314.00
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Monoclonal NGF/proNGF Neutralizing Antibody Antibody

AMM06679G 0.05mg
EUR 528
Description: A Monoclonal antibody against Human NGF/proNGF Neutralizing. The antibodies are raised in Mouse. This antibody is applicable in E

Anti CD22 Antibody: CD22 Monoclonal Antibody

065-A-01mg 0,1 mg
EUR 267.5
Description: anti-CD22 monoclonal antibody

Anti CD22 Antibody: CD22 Monoclonal Antibody

065-A-1000ug 1000 ug
EUR 1282.5
Description: anti-CD22 monoclonal antibody

Anti-IgG1 Negative Control Antibody antibody

STJ16100881 1 mL
EUR 478

Anti-Noelin Antibody BIOTIN Antibody BIOTIN

STJ501938 100 µg
EUR 586

Moreover, we confirmed that roots of rye crops took up compounds originating from neighbouring crops. Among the many compounds taken up by rye roots, wogonin was detected within the rye shoot, which indicated a root-to-shoot translocation of this compound.

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